When absolute accuracy is critical

How much protein is there? It may seem an easy question, but in practice protein quantification is a difficult task. Being a sensitive measurement, large errors are easy to make and there are only a limited number of accurate methods available.

We’ve put a lot of work into developing and optimising the methods we use. Now, we offer our own specially developed techniques that give high precision measurements and accuracy.

Furthermore, we cross-validate our methods so that you don’t have to just take our word for it. We can show that our methods do what we said they would.

So if you need to know the precise quantity of a protein in your drug development or research project, you can choose from the following services.

• Amino Acid Analysis (AAA), to determine the amino acid composition of a protein without using an external standard.
• Extinction Coefficient (A280), to calculate protein concentration by measuring UV absorbance at A280. This technique helps demonstrate consistency and comparability between batches.

Note that protein concentration can also be determined by ELISA assays or mass spectrometry using absolute quantification (AQUA).

Protein quantification is a key part of ICH Q6B.

Your contact for Protein Quantification at Protagen AG

Dr Katja Aschermann Head of Business Development Protein Services. Her team will be happy to assist you with your project. Phone: +49 (0) 231 9742-6300 Email: salesproteinservices@protagen.de